Biolistic-mediated transformation - msu edu


    fAdvantage: yThis method can be use to transform all plant species. yNo binary vector is required. yTransformation protocol is relatively simple. fDisadvantage: yDifficulty in obtaining single copy transgenic events. yHigh cost of the equipment and microcarriers. yIntracellular target is random (cytoplasm, nucleus, vacuole, plastid, etc.). yTransfer DNA is not protected.


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